Biocatalytic synthesis of the Green Note trans-2-hexenal in a continuous-flow microreactor

• Morten M. C. H. van Schie,
• Tiago Pedroso de Almeida,
• Gabriele Laudadio,
• Florian Tieves,
• Elena Fernández-Fueyo,
• Timothy Noël,
• Isabel W. C. E. Arends and
• Frank Hollmann

Beilstein J. Org. Chem. 2018, 14, 697–703, doi:10.3762/bjoc.14.58

• -enol using a novel aryl alcohol oxidase from Pleurotus eryngii (PeAAOx) is reported. As O2-dependent enzyme PeAAOx-dependent reactions are generally plagued by the poor solubility of O2 in aqueous media and mass transfer limitations resulting in poor reaction rates. These limitations were efficiently

• overcome by conducting the reaction in a flow-reactor setup reaching unpreceded catalytic activities for the enzyme in terms of turnover frequency (up to 38 s−1) and turnover numbers (more than 300000) pointing towards preparative usefulness of the proposed reaction scheme. Keywords: alcohol oxidase

• catalytic amounts only) causes additional costs. Therefore, we concentrated on alcohol oxidase-catalysed reaction schemes (Scheme 1B. Oxidases utilise O2 as terminal electron acceptor for the oxidation reaction yielding H2O2 as sole byproduct. The latter can be disproportionated easily by using catalase

Engineering Pichia pastoris for improved NADH regeneration: A novel chassis strain for whole-cell catalysis

• Martina Geier,
• Christoph Brandner,
• Gernot A. Strohmeier,
• Mélanie Hall,
• Franz S. Hartner and
• Anton Glieder

Beilstein J. Org. Chem. 2015, 11, 1741–1748, doi:10.3762/bjoc.11.190

• oxidized by alcohol oxidase (AOX) to formaldehyde, which is further metabolized either in the assimilatory or in the dissimilatory pathway. In the latter one, formaldehyde spontaneously reacts with glutathione to S-hydroxymethylglutathione which is oxidized in a first step by the glutathione- and NAD

• )-acetoin: 2.32 min, (2R,3R)-butane-2,3-diol: 8.27 min, meso-butane-2,3-diol: 8.90 min. Simplified schematic representation of the methanol utilization pathway in Pichia pastoris. The main pathways and the respective enzymes are shown. AOX: alcohol oxidase; FLD: glutathione-dependent formaldehyde